Effects of tempol on renal medullary tissue hypoxia in an ovine model of Gram-negative septic acute kidney injury

Abstract

Renal arterial infusion of tempol (RAT) at the onset of Gram-negative sepsis can prevent sepsis-induced medullary tissue hypoxia and acute kidney injury (AKI). However, it is not known whether treatment with tempol at a clinically relevant time point of sepsis is similarly effective. Thus, we examined whether tempol can reverse renal medullary tissue hypoxia after ovine Gram-negative septic AKI. Following right unilateral nephrectomy, the left kidney was instrumented with a renal arterial catheter and oxygen-sensing fibre-optic probes into the renal medulla. After 23 h of Escherichia coli infusion, conscious sheep were fluid resuscitated with Hartmann's solution (30 mL/kg over 0.5 h) and ran..